![]() Even though lentivirus is the customary method, there is still hesitation because an extensive safety profile is still being established and understood. ![]() That is why there is an immense interest in a delivery technology that will increase flexibility and improve delivery efficiencies. The Neon Electroporation system is an easily adaptable solution in which successful delivery of mRNA in primary T-cells has been shown to achieve greater than 75% efficiency. With minimal optimization, we will show how the Neon system can be used for immune cell-based applications. XĪttune NxT Flow Cytometer with Automated Acquisition Sampler as a High Throughput Screening Tool – Multiplexed Flow Cytometry Used to Analyze Metabolic Activity and Assess Pharmaceutical Compound Toxicity These technologies can be applied to help advance research within immunology, immunotherapy, and immuno-oncology. Quentin Low, Staff Scientist, Life Sciences Group, Thermo Fisher Scientificįlow cytometry has recently emerged as a powerful HTS tool adding the benefits of single cell multiparametric analysis.We compared multiplex flow cytometer and single-read plate reader HTS platforms by screening drug compound libraries on Jurkat and Ramos cell lines. #Seattle medical conferences 2016 seriesĬells were cultured under either normoxic (5%) or hyperoxic (19%) conditions grown in the presence of the Microdiscovery Discovery “Killer Plate” drug compound library under a series of time points (24, 48, or 72 hrs). The two oxygen levels simulate cellular oxygen conditions in either a tumor environment or normal cellular environment. ![]() #Seattle medical conferences 2016 series.
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